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Objective:To optimize the chitosan flocculation purification process of Zhigancao decoction. Methods:With the total content of ginsenoside Rg1 , Re and Rb1 and the impurity removal rate as the evaluation indices, an orthogonal test was used to investi-gate the effects of concentration of solution, amount of chitosan and standing temperature on the purification process. Results:The op-timum conditions of chitosan flocculation clarification process of Zhigancao decoction were as follows:the concentration of extraction so-lution was 0. 25 g·ml-1 , the standing temperature was 25℃, and the amount of chitosan was 12% . Conclusion:The optimum con-ditions of purification process are promising with high impurity removal rate and active ingredients retention rate, which provide refer-ence for the dosage form reformation of Zhigancao decoction.
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Objective:To explore the effects of holly leaf extract on blood lipid regulating function and blood rheology in hyperlipi-demia rats to provide experimental basis for the development and application of holly leaf. Methods:The rats were fed with high-fat di-et for 4 weeks to establish the model of hyperlipidemia. The rats were randomly divided into the control group, the model group, the positive group, high-dose group (0.60 g·mL-1), medium-dose group (0.30 g·mL-1) and low-dose group (0.15 g·mL-1) of Holly leaf with gastric perfusion for 9 weeks. TC, TG, HDL-C, LDL-C, AST, ALT, SOD and MDA in serum of the rats were deter-mined, and various indices of hemorheology were measured. Results:When compared with the model group, holly leaf extract at dif-ferent doses could decrease the content of TC, TG, LDL-C, ALT, AST, MDA and apo B in varying degrees, and increase the content of HDL-C, the SOD activity and lipoprotein levels in varying degrees at the end of the gastric perfusion, and the effects of high-dose of holly leaf were the most significant (P<0. 05,P<0. 01). Holly leaf extract at different doses could decrease plasma viscosity, whole blood viscosity, erythrocyte aggregation index and hematocrit, and increase red blood cell deformability index, and the effects of high-dose group of holly leaf were the most significant (P<0. 05,P<0. 01). Conclusion:Holly leaf has obvious regulating effects on blood lipid function and blood rheology in hyperlipemia rats, which can reduce blood lipid levels.
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Objective To compare the effects oflightwand(LW) and direct laryngoscope(DL) in elderly patients undergoing surgery on hemodynamics and stress responses.Methods Forty elderly patients with ASA grade Ⅰ-Ⅱ grade who selective surgery were divided into LW group and DL group by random digits table method,each group with 20 cases.Systolic blood pressure (SBP),diastolic blood pressure (DBP),heart rate(HR) were recorded respectively before intubation,30 seconds and 5 minutes after intubation.Extracted patients with peripheral arterial 3 ml at the same time,measured by radioimmunoassay epinephrine and norepinephrine levels.Results The SBP,DBP,HR,epinephrine,norepinephrine 30 seconds after intubation in LW group were significantly lower than those in DL group [(140.50 ± 21.91) mm Hg(1 mm Hg =0.133 kPa) vs.(167.95 ± 17.85) mm Hg,(67.80 ± 6.76) mm Hg vs.(84.15 ±9.89) mm Hg,(85.10 ± 11.76) times/min vs.(96.30 ± 13.72) times/min,(49.47 ± 8.09) ng/L vs.(61.25 ± 9.43) ng/L,(171.27 ± 17.11) ng/L vs.(187.40 ± 16.13) ng/L,P ＜ 0.01].The SBP,epinephrine,norepinephrine 5 minutes after intubation in LW group were significantly lower than those in DL group [(120.75 ± 17.12) mm Hg vs.(136.65 ± 15.43) mm Hg,(39.80 ±7.38) ng/L vs.(47.63 ±8.48) ng/L,(155.93 ± 17.11) ng/L vs.(172.76 ± 13.45) ng/L,P ＜ 0.01].Conclusion Both LW and DL can be well done in elderly patients intubation,but LW can reduce stress responses during the intubation.